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DOT Urine Colelctor & EBT/BAT Refresher Training

Table of Content:

What is Refresher Training?

Why do specimen collectors need refresher training?

Why is refresher training important?

How is refresher training conducted?

Enroll in a refresher training course


Specimen collectors who conduct drug tests and Breath Alcohol Technicians (BATs) who conduct alcohol tests are essential to ensuring safety in the workplace. This is why these professionals are required to complete mandatory refresher training within 5-years of becoming qualified. Qualified specimen collectors and BATs often provide drug and alcohol testing services to both DOT and Non-DOT regulated companies. DOT-regulated companies may be regulated by one or more DOT subagencies:

  • Federal Motor Carrier Safety Administration (FMCSA)
  • Federal Railroad Administration (FRA)
  • Federal Aviation Administration (FAA)
  • Federal Transit Administration (FTA)
  • Pipeline and Hazardous Materials Safety Administration (PHMSA)
  • United States Coast Guard (USCG)

To qualify as a urine specimen collector under United States Department of Transportation regulations, for example, DOT collectors must abide by all of the requirements outlined in 49 C.F.R. § 40.33. Critical to compliance is ensuring ongoing refresher training every five years. This additional training is designed to ensure that urine specimen collectors stay up-to-date on the regulations and are reminded of collection procedure requirements. This same principle applies to all refresher training. If DOT collectors and BATs fail to complete refresher training they will find themselves no longer qualified to conduct drug and alcohol testing.

Non-DOT Urine Specimen Collections are regulated by state law rather than federal law. Contrary to popular belief, DOT qualification does not carry forward to Non-DOT qualification. Many state laws include collection and testing practices that differ greatly from federal regulations and thus require individualized training. Drug and alcohol testing laws are constantly changing under state law, so in addition to Non-DOT refresher training courses, we recommend enrolling in one of our updated state-specific workplace testing laws courses. 

Requirements of DOT Refresher Training

DOT Urine Specimen Collector refresher training involves two out of the three initial training requirements. Instead of completing modules on basic information, qualification training, and initial proficiency demonstrations, refresher training mandates only qualification training and initial proficiency demonstrations.

Refresher training courses will include a review of CCF (Custody and Control Form) completion and remittance, identity verification procedures, and problem collections such as shy bladder and direct observation procedures.

Finally, the refresher course will involve five error-free mock collections. These scenarios will be:

  • 2 uneventful collections
  • 1 collection with an inadequate amount of urine
  • 1 collection where the temperature is out-of-range
  • 1 incident where the person refuses to sign the CCF and/or put their initials on the specimen bottles tamper-evident seal

All of these mock collections will be carried out under the supervision of a qualified instructor, known as a monitor.

Similarly, the DOT Breath Alcohol Technician training course involves qualification training and initial proficiency demonstrations, but not basic information. Unlike DOT Urine Specimen Collector Refresher Training, DOT Breath Alcohol Technician Refresher Training requires the completion of 7 error-free mock demonstrations. The DOT does not mandate the particular scenarios which must be completed.

Staying Current on Any Changes to Federal Regulations

Just as state law is subject to change, federal law frequently changes as well. All service agents including DOT Urine Specimen Collectors and DOT Breath Alcohol Technicians must stay up-to-date on all applicable federal drug and alcohol testing regulations including the 49 C.F.R. § 40 in the time period between completion of initial training and refresher training.

Non-DOT Specimen Collector Refresher Training

Drug and alcohol testing laws are constantly changing. We recommend enrolling in a Workplace Drug Testing Law course annually for specimen collectors who perform drug testing using oral saliva/fluid, hair, and Non-DOT Urine. To begin, select the state in which you are currently conducting drug and alcohol testing. For questions, please contact 1-888-390-5574.


If you have any additional questions, send us an email to [email protected] or give us a call at 1-888-390-5574.

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