DOT Oral Fluid Specimen Train-the-Trainer



Course Overview

This course will qualify you to train others to collect oral fluid specimens in accordance with a federal Drug-Free Workplace Program.

Section 4.4 of the Substance Abuse and Mental Health Administration (SAMHSA) Oral Fluid Mandatory Guidelines (OFMG) provides the requirements for those who would train others to collect saliva specimens under federal protocols.

Specifically, the OFMG provides that in order for a collector trainer to be qualified to train others, the prospective trainer may enroll in a “train the trainer” course and then must complete collector refresher training every five years and must maintain record of training which must be available upon request.

Our Train-the-Trainer courses are designed to exceed basic skills learned in the DOT / HHS Oral Fluid Specimen Collector Course and ensure that trainers are qualified to prepare others to perform oral fluid collections under federal protocols.

A trainer’s understanding of the collection process must go far beyond the initial training requirements for collectors and include a thorough understanding of the regulations, problems in collection, location of helpful resources, and contact information for relevant agency guidance when necessary.

Successful trainers will be able to teach and verify retention of required knowledge for collectors as outlined in the regulations, be knowledgeable about every federal agency under which collectors will be trained, and be intimately familiar with the oral fluid collection device or devices that will be used to train others. Successful trainers will also know each step of the collection process, the appropriate method of completion and distribution of the Federal Custody and Confrol Form (CCF), be familiar with common and uncommon problem collections, fatal flaws, and methods for correction of problem collections.

Most importantly, successful trainers will be able to adequately convey to collector trainees the role of the collector and the responsibility for maintaining the integrity of the collection process, privacy of the donor, security of the specimen, and how to avoid conduct or statements that could be viewed as offensive or inappropriate.

If you would like to become qualified to simply collect oral fluid/saliva specimens rather than train others to do so, enroll in our DOT / HHS Oral Fluid Specimen Collector Course instead.

This course meets or exceeds the training requirements in all 50 states and for the following federal agencies:

  • Federal Motor Carrier Safety Administration (FMCSA),
  • Federal Aviation Administration (FAA),
  • Federal Transit Administration (FTA),
  • Federal Railroad Administration (FRA),
  • United States Coast Guard (USCG), and
  • Pipeline and Hazardous Materials Safety Administration (PHMSA).
Minimum Technology Requirements
  • A computer or mobile device that is less than 5 years old.
  • Speakers, headphones, or earbuds to listen to audio
  • Webcam for interacting with instructors during mock collection (this only applies to courses with mock collections)
  • Internet Browsers, such as Mozilla Firefox and Google Chrome, are preferred to download these browsers visit Mozilla Firefox (latest version) – Download or Google Chrome (latest version) – Download
  • Adobe Acrobat Reader(latest version) can be used for viewing PDF files offline (outside of a web browser). – 
  • A stable high-speed Internet connection or greater is required.
  • If using Internet Explorer, please upgrade to Microsoft Edge since Microsoft has discontinued the use of Internet Explorer.

Course Features & FAQ


DOT Compliant

This course meets or exceeds the training requirements in all 50-states, the following federal agencies: FMCSA, FAA, FTA, FRA, USCG, PHMSA, and other counties that use the DOT guidelines.

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Created by Attorneys

This course and its supplemental resources were created by our co-founder, attorney, and drug and alcohol testing authority and subject matter expert, Andrew David Easler, Esq.

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Live Customer Support

Students, managers and supervisors can contact us, chat with a us-based trainer to answer questions for the duration of the course by calling 1-888-390-5574, using our in-application live chat, or sending an email and we will respond within 60-minites during of regular business hours.

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Learn Anytime & Anywhere

Our courses only expire when you're finished with the course, this means that employees don’t feel rushed into “cramming” for a course only to forget the material a week later and therefore perform better in practice and make fewer mistakes than in traditional training environments.


Credits Never Expire

Unused course credits never expire, so feel free to order bulk credits to receive maximum discounts.

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Instant Course Access

Employees receive instant course access right after registration or invitation sent by a training manager, or supervisor.


4-Hours of Learning

DOT Oral Fluid Specimen Train-the-Trainer may take longer or shorter to complete depending on student pace, reading, and comprehension level.
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Downloadable Certificate

Same-day certificate issuance after completing a course, downloadable in PDF format & printable.

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Instant Manager Accounts

When a employer purchases two or more credits we will automatically create a manager account which can then be used to invite employee, keep all training certificates in one place that will help you stay compliant and well prepared for a audit conduct by the DOT.

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Volume Discounts

Volume discounts for credits are automatically applied during the checkout process. Did you know that course credits never expire so feel free to purchase credits in bulk.


Approved for CE Credits

We are an Approved Education Provider with many human resources certification organizations, licensing bodies and regulatory agencies throughout the world and we offer continuing education credits (CE Credits) with the majority of our courses.

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Additional Resources

Employers can purchase training and reference books which will assist students and supervisors in understanding the regulations involved for each applicable DOT sub agency including the FMCSA, FAA, FRA, FTA, PHMSA and USCG.

Legal Research on DOT Oral Fluid Specimen Train-the-Trainer